Question: Can I use wide tissue slides (26 mm width) with the Visium CytAssist Spatial Gene Expression assays?
Answer: No, the Visium CytAssist Spatial Gene Expression assays are not compatible with 26 mm width tissue slides. Our Tissue Slide Cassettes (supplied in Visium CytAssist Spatial Gene Expression kits) currently support glass slides with the maximum dimensions specified in Table 1 below.
| Length (mm) | Width (mm) | Thickness (mm) | |||
| Minimum | Maximum | Minimum | Maximum | Minimum | Maximum |
| 74.4 | 76.2 | 24.8 | 25.3 | 0.96 | 1.07 |
| Table 1. Minimum and maximum length, width, and thickness of glass slides the Visium CytAssist Tissue Slide Cassette supports. | |||||
Listed in Table 2 are specific brands of glass slides that have been identified to not fit Visium CytAssist Tissue Slide Cassettes. Use of these slides is strongly discouraged as they may impact assay performance due to slide breakage and/or cassette leakage.
| Slide Brand | Slide Name |
Catalog Number |
| Leica | Permaflex | |
| Permaflex Plus | ||
| X-tra | ||
| Matsunami | Platinum Pro | |
| Muto-Kagaku | Star Frost Glass | |
| Trajan | Series 2 Adhesive | |
| Table 2. Glass slide brands identified as incompatible with Visium CytAssist Tissue Slide Cassettes. Refer to the appropriate Tissue Preparation Guide for validated brands of glass slides. | ||
For more details, see the following resources:
- What glass slides are compatible with the Visium CytAssist for FFPE assay?
- Visium CytAssist Spatial Gene Expression for FFPE Tissue Preparation Guide
- Visium CytAssist Spatial Gene Expression for FF Tissue Preparation Guide
If interested in processing tissue sections on 26 mm wide slides, the Visium HD Spatial Gene Expression assay is compatible with both 25 mm and 26 mm wide glass slides. Please see the following article for more details: What glass slides are compatible with the Visium HD assay?
Products: Visium CytAssist for FFPE, Visium CytAssist for FF, Visium CytAssist for FxF