Question: What fixation and embedding methods are compatible with Xenium using FFPE tissue inputs?
Answer: Xenium for FFPE should be used with human or mouse tissue fixed in a crosslinking formaldehyde solution; such as 10% neutral buffered formalin (NBF) or 4% paraformaldehyde (PFA), and then embedded in paraffin wax. Alternative fixation or embedding methods are unsupported. If tissue samples are fresh, we would recommend proceeding with fresh frozen sample preparation rather than fixation and embedding.
Product: Xenium In Situ Gene Expression