Question: Which thermal cyclers are compatible with Xenium?
Answer: The following thermal cycler has been validated to be compatible with Xenium In Situ Gene Expression by 10x Genomics:
- Bio-Rad C1000 Touch™ Thermal Cycler with 96-Deep Well Reaction Module (Cat No. 1851197)
- Analytik Jena Biometra TAdvanced 96 SG (846-x-070-241) or base unit (846-x-070-280)
- Thermo Fisher Scientific Veriti (REF: 4375786)
- VWR xt96 Gradient (76452-153)
- MJ Research PTC-200 (05434-05)
1) Lid application
The adjustable thermal cycler lid allows for proper sealing of the cassette lid over the sample well.
2) Two Xenium slides in cassettes with lids sitting on Xenium thermocycler adaptor
The deep-well block allows the Xenium Thermocycler Adaptor to sit directly on top of the block for efficient heat transfer to the Xenium slide. Not using a deep-well block will lessen the heat transfer from the block to the slide and result in a decrease in assay performance.
3) Xenium Thermocycler Adaptor
Thermal Cycler best practices for C1000:
If using thermal cyclers other than BioRad C1000, ramp rates should be adjusted for all the steps as described below:
- Analytik Jena Biometra TAdvanced: 2°C/sec heating and cooling
The lid temperature of some of the validated thermal cyclers do not go lower than 50°C, but some steps of the Xenium workflow calls for lower lid temperatures. For those steps that require lower lid temperatures, we would advise turning off the lid heating but keeping the lid closed during incubation. Are thermal cyclers with lid temperature that do not go lower than 50°C compatible with Xenium?
Additional thermal cyclers are untested and therefore we cannot guarantee assay performance.
Please refer to support materials for tips and best practices:
- Xenium In Situ for FFPE Tissues – Deparaffinization & Decrosslinking
- Xenium In Situ for Fresh Frozen Tissues – Fixation & Permeabilization
- Xenium In Situ Gene Expression - Probe Hybridization, Ligation & Amplification
Products: Xenium In Situ Gene Expression