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Which thermal cyclers are compatible with Xenium?

Question: Which thermal cyclers are compatible with Xenium?

Answer: The following thermal cyclers have been validated by 10x Genomics for use with Xenium In Situ Gene Expression as outlined on the support webpage thermal cycler compatibility table.

 

Xenium v1

  • Analytik Jena Biometra TAdvanced 96 SG (846-x-070-241)(where x=2 for 230 V; 4 for 115 V; 5 for 100 V, 50-60 Hz)
  • Analytik Jena Biometra TAdvanced 96 S (846-x-070-251)(where x=2 for 230 V; 4 for 115 V; 5 for 100 V, 50-60 Hz)
  • Bio-Rad C1000 Touch™ Thermal Cycler with 96-Deep Well Reaction Module (Cat No. 1851197)(discontinued)
  • VWR XT96 Gradient (76452-153)
  • MJ Research PTC-200 (05434-05)

Here is a list of thermal cyclers that were tested internally and found to be incompatible with Xenium v1. Please note, failure modes listed below are usually intermittent. For example, slides might only crack ~20% of the time. Reduced sensitivity may not impact every run or the entire tissue section.

Thermal Cycler* Phenotype
Mastercycler x50
  1. Poor cassette lid sealing.
  2. Slide evaporation --> Reduced sensitivity and potential for assay failure.
PTC Tempo
  1. Slides cracked during overnight Probe Hyb.
  2. Cassette clips bent --> Reduced sensitivity and potential for assay failure.
  3. Lid error with Xenium cassettes.
Proflex 96W
  1. Slides cracked during overnight Probe Hyb.                        
T100
  1. Reduced sensitivity and potential for assay failure.
Veriti
  1. Slides cracked during overnight Probe Hyb.
  2. Veriti lid hinges cracked when lid closed.
Veriti Pro
  1. Slides cracked during overnight Probe Hyb.

*Thermal cyclers that are not included in the list above are not tested.

 

Xenium Prime

  • Analytik Jena Biometra TAdvanced 96 SG (846-x-070-241)(where x=2 for 230 V; 4 for 115 V; 5 for 100 V, 50-60 Hz)
  • Analytik Jena Biometra TAdvanced 96 S (846-x-070-251)(where x=2 for 230 V; 4 for 115 V; 5 for 100 V, 50-60 Hz)
  • Bio-Rad C1000 Touch™ Thermal Cycler with 96-Deep Well Reaction Module (Cat No. 1851197)(discontinued)
  • Bio-Rad PTC Tempo Deepwell Thermal Cycler (12015392)
  • ThermoFisher Scientific VeritiPro 96-well Thermal Cycler (A48141)
  • Eppendorf Mastercycler X50s (6311000010)
  • Eppendorf Mastercycler X50a (6313000018)

Here is a list of thermal cyclers that were tested internally and found to be incompatible with Xenium Prime. Please note, failure modes listed below are usually intermittent. 

Thermal Cycler* Phenotype
T100
  1. Poor performance.
XT96
  1. Validation experiments resulted in high variance in evaporation tests as well as assay sensitivity performance tests.

 

For all unsupported models, we cannot guarantee assay performance or offer replacements for unexpected gene expression or cell segmentation staining for experiments run on these. Unsupported thermal cyclers may be incompatible with the Xenium Slide Cassettes, leading to cassette leakage or evaporation, broken slides, and reduced assay sensitivity as compared to validated thermal cyclers. These phenotypes are most often observed during overnight incubation and high-temperature steps.

 

! Always ensure the appropriate Xenium Thermocycler Adaptor is used:

Chemistry Item
Xenium v1

Xenium Thermocycler Adaptor (PN-1000623)
Xenium Prime Xenium Thermocycler Adaptor v2 (PN-1000739)

 

Product: Xenium In Situ Gene Expression, Xenium Prime In Situ Gene Expression