Question: Is drop dialysis necessary during gel plug DNA extraction?
Answer: When extracting genomic DNA using the gel plug method (for example, Bionano Prep™ Plant Tissue DNA Isolation Base Protocol: https://bionanogenomics.com/wp-content/uploads/2017/01/30068-Bionano-Prep-Plant-Tissue-DNA-Isolation-Protocol.pdf) there is a drop dialysis step after recovering DNA from the gel plug. The drop dialysis step is used to remove residual contaminants and enzymes from the sample, which could inhibit DNA amplification.
In the 10x Genomics Genome/Exome protocols the gDNA sample is typically diluted significantly (100 fold or more) to arrive at a 1 ng/ul solution for loading in the 10x Sample Master Mix. Therefore, when the gDNA is diluted to this extent, we do not perform the drop dialysis step. However, if the gDNA sample is diluted less than 100 fold to arrive at the 1 ng/ul concentration, we would recommend performing the drop dialysis step to minimize any contaminants going into the Sample Master Mix.
Products: Genome, Exome