Question: What are recommended control B cells for V(D)J assay?
Answer: Below are recommended control B cells that can be used in the Single Cell 5' VDJ assay:
- Normal Peripheral Blood CD19+ B Cells - AllCells (Catalog #PB010F), cryopreserved, 10 million cells, negatively isolated from mononuclear cells with magnetic beads
Please see the 10x Genomics Demonstrated Protocol 'Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing' for general guidelines on cell thawing, washing and preparation: https://support.10xgenomics.com/single-cell-vdj/index/doc/demonstrated-protocol-fresh-frozen-human-peripheral-blood-mononuclear-cells-for-single-cell-rna-sequencing
Products: V(D)J