Question: How do I extract genomic DNA from tissue samples?
Answer: We recommend using SPRI bead-based DNA purification methods for tissue samples. We have a Demonstrated Protocol for obtaining HMW gDNA from fresh frozen tissue using SPRI beads (https://support.10xgenomics.com/genome-exome/sample-prep/doc/demonstrated-protocol-hmw-dna-extraction-from-fresh-frozen-tissue). This method can produce gDNA with an average size of >200 kb when analyzed on a pulsed-field gel. After the Chromium Genome Assay, the average size of the library inserts is >80 kb as measured by Long Ranger.
Optimization may be required for each tissue type or sample source.
Note: Heavily damaged DNA, such as DNA extracted from FFPE tissue, will not perform well in the Chromium Genome assay due to the short molecule size.
Products: Genome, Exome