Question: How do I extract genomic DNA from saliva?
Answer: We validated the PerkinElmer chemagic 360 nucleic acid extractor (http://www.chemagen.com/chemagic-360/) for saliva HMW gDNA extraction. The chemagen automated technology uses magnetic beads to isolate and purify nuclei acid and magnetizable rotating rods produce long and ultra-pure DNA fragments. The chemagic DNA Saliva Kit is optimized for saliva collected using DNA Genotek's Oragene sample collection tubes.
We have not tested non-automated kits for gDNA extraction from saliva, however, customers have made successful Genome libraries from saliva gDNA using the Mawi (http://www.mawidna.com/products/iswab-dna-collection-kit/) kit for sample collection and a magnetic bead-based method for DNA purification. After the Chromium Genome Assay, the average size of the library inserts is ~20 kb as measured by Long Ranger.
It is important to keep in mind that stabilized saliva solution contains a number of different cell states, including intact heathy cells, dying/dissociated cells, and free-floating DNA, therefore the quality of genomic DNA extracted from saliva will be lower than other starting sample types.
Products: Genome, Exome