Question: Why do I need an extra kit if I want Gene Expression and/or Feature Barcoding in addition to V(D)J?
Answer: To generate Single Cell 5' Gene Expression library and one V(D)J enriched library (either T or B enriched) from a single sample, an additional Chromium Single Cell 3'/5' Library Construction Kit (16 rxns; PN-1000020) is needed. This is because the Chromium Single Cell 5' Library and Gel Bead Kit (PN-1000006 or PN-1000014) only contains enough reagents for generating one sequencing library per sample.
If you need to generate Single Cell 5' Gene Expression library and two V(D)J enriched libraries (T- and B) from a single sample (three sequencing libraries per sample), two Chromium Single Cell 3'/5' Library Construction Kit (16 rxns; PN-1000020) are needed in addition to the Chromium Single Cell 5' Library and Gel Bead Kit (PN-1000006 or PN-1000014).
If you need to generate a Feature Barcoding library in addition to the V(D)J enriched libraries and/or Gene Expression library you will also need to a Feature Barcode Library Kit (PN-1000080).
Products: V(D)J