Question: Why do I need an extra kit if I want Gene Expression and/or Feature Barcoding in addition to V(D)J?
Answer: To generate Single Cell 5' Gene Expression library and one V(D)J enriched library (T or B cell-enriched) from a single sample, an additional Chromium Single Cell 5' Library Construction Kit (16 rxns; PN-1000020) is needed. This is because the Chromium Single Cell 5' Library and Gel Bead Kit only contains enough reagents for generating one sequencing library per sample.
If you need to generate a Single Cell 5' Gene Expression library and two V(D)J enriched libraries (T and B enriched) from a single sample, you will be making 3 libraries so keep in mind how many samples and kits you may need to purchase.
If you need to generate a Feature Barcoding library in addition to the V(D)J enriched libraries and/or Gene Expression library, you will also need to a Chromium Single Cell 5' Feature Barcode Library Kit (PN-1000080) and a Chromium i7 Multiplex Kit N, Set A (PN-1000084).
Products: Single Cell Immune Profiling