Question: Why are Exome libraries sheared to 225 bp?
Answer: For generating Exome libraries, we recommend shearing the post-GEM incubation product to ~225 bp. This is because Agilent recommends a target DNA fragment size between 150 to 200 bp for efficient capture using the Agilent SureSelectXT Human All Exon V6 Kit. An additional 22 bp represents the 10x barcode and N-mer.
Product: Exome