Question: Can I study T and/or B cell immune repertoires along with profiling gene expression from the same cells?
Answer: Yes. The Single Cell V(D)J Solutions offer the option to generate: an enriched T cell library and/or an enriched B cell library, and/or a 5’ gene expression library from amplified cDNA from the same cells
If you are interested in 5' gene expression profiling alongside T and/or B cell immune repertoires, you will need to use Option 2. This will involve one round of cDNA amplification prior to T and/or B cell enrichment and requires an additional library kit for each additional sequencing library you would like to generate from a given sample (Chromium™ Single Cell 3'/5' Library Construction Kit).
Below are the reagent kits needed for cDNA amplification followed by Target Enrichment
Note: If you are interested in generating a 5' gene expression library and both T and B cell enriched libraries, two Chromium™ Single Cell 3'/5' Library Construction Kits are necessary.
For additional details, see https://support.10xgenomics.com/single-cell-vdj/library-prep/doc/user-guide-chromium-single-cell-vdj-reagent-kit