Question: I have less than 50 ng cDNA. Can I still make a 5' Gene Expression library?
Answer: Yes, if the cDNA in 20 ul is less than our recommended mass for library construction (50 ng), it is possible to increase the volume of cDNA added to the fragmentation reaction from 20 ul to 35 ul, omitting 15 ul of water in the Fragmentation Mix. If proceeding this route, the total mass of cDNA should be at least 2 ng.
Products: Single Cell Immune Profiling