Question: What does the Productive column mean in the
contig_annotations.csv file generated by
Answer: The Productive column in the
filtered_contig_annotations.csv) identifies contigs that are predicted to generate functional proteins. For a contig to be marked as productive it must:
- Be V-J spanning with a start codon in the leader region.
- Have a detectable CDR3 region in frame with the start codon.
- Be free of stop codons in the V-J spanning region.