Question: What Illumina sequencers are supported for Single Cell V(D)J libraries?
Answer: The following instruments are supported for sequencing V(D)J libraries:
- Illumina® NovaSeq
- Illumina® HiSeq 3000/4000
- Illumina® HiSeq 2500 Rapid Run and High Output
- Illumina® NextSeq 500/550*
- Illumina® MiSeq
*Sequencing Single Cell V(D)J libraries with the NextSeq 500/550 v2 kit may yield reduced sequence quality and sensitivity relative to the MiSeq and HiSeq platforms. There have been recent reports of improved sequencing quality with NextSeq v2.5 sequencing kits.
For more information on sequencing metrics for 10x Genomics libraries, see the related Technical Note: https://support.10xgenomics.com/single-cell-vdj/index/doc/technical-note-chromium-single-cell-vdj-libraries-sequencing-metrics-for-illumina-sequencers
Products: Single Cell Immune Profiling