Question: What Illumina sequencers are supported for Single Cell V(D)J libraries?
Answer: The following instruments are supported for sequencing V(D)J libraries:
- Illumina® NovaSeq
- Illumina® HiSeq 3000/4000
- Illumina® HiSeq 2500 Rapid Run and High Output
- Illumina® NextSeq 500/550*
- Illumina® MiSeq
*Sequencing Single Cell V(D)J libraries on the NextSeq 500/550 platform may yield reduced sequence quality and sensitivity relative to the MiSeq and HiSeq platforms.
For more information on sequencing metrics for 10x Genomics libraries, see the related Technical Note: https://support.10xgenomics.com/single-cell-vdj/index/doc/technical-note-chromium-single-cell-vdj-libraries-sequencing-metrics-for-illumina-sequencers