Question: Can the Single Cell V(D)J kit be used to amplify non-human/mouse T- or B-cell receptors?
Answer: The Single Cell V(D)J kit contains species-primers designed to enrich either human or mouse T-cell or B-cell receptor genes. Therefore, the kit is not suitable for use with non-human samples. However, it is possible to design custom enrichment primers to amplify non-human receptors in place of the primers provided by 10x Genomics, although these applications are not supported.
For guidance, we recommend consulting the 'Oligonucleotide Sequences' in our appendix of the appropriate User Guide, which details the Single Cell V(D)J assay scheme and primer sequences for the enrichment primers.
The following article may also be helpful for designing enrichment primers for custom species: How can I optimize V(D)J primer design for my non-human/non-mouse species of interest?
Products: Single Cell Immune Profiling