Question: Can the Single Cell V(D)J kit be used to amplify non-human T- or B-cell receptors?
Answer: The Single Cell V(D)J kit contains human-specific primers to enrich human T-cell or B-cell receptor genes. Therefore, the kit is not suitable for use with non-human samples. However, it is possible to design custom enrichment primers to amplify non-human receptors in place of the primers provided in the Human or Mouse T Cell Mix 1 & 2 or Human or Mouse B Cell Mix 1 & 2, although these applications are not currently supported.
For guidance, we recommend consulting the following User Guide, which details the Single Cell VDJ assay scheme, primer sequences, as well as primer concentrations for enrichment primers: