Question: Are Microbeads (Miltenyi Biotec) and EasySep beads (STEMCELL) compatible with the 10x Single Cell RNA-seq workflow?
Answer: The Miltenyi Biotec Microbeads are fully compatible with the Chromium Controller and Single Cell 3' and Single Cell V(D)J solutions, both for positive and negative selection of cells. They are small enough that they do not interfere with partitioning or emulsion stability. We have several Demonstrated Protocols using Microbeads with the 10x system:
- Enrichment of CD3+ T cells from dissociated tissue: https://support.10xgenomics.com/single-cell-gene-expression/index/doc/demonstrated-protocol-enrichment-of-cd3-t-cells-from-dissociated-tissues-for-single-cell-rna-sequencing-and-immune-repertoire-profiling.
- Removal of dead cells from single cell suspensions: https://support.10xgenomics.com/single-cell-gene-expression/index/doc/demonstrated-protocol-removal-of-dead-cells-from-single-cell-suspensions-for-single-cell-rna-sequencing.
The EasySep beads from STEMCELL have not specifically been tested for compatibility with the Chromium Controller and Single Cell 3' and Single Cell V(D)J solutions. However, these beads have very similar properties to the Miltenyi Microbeads microbeads.
Products: Single Cell Gene Expression, Single Cell Immune Profiling