Question: What are recommended control T cells for V(D)J assay?
Answer: Below are recommended control T cells that can be used in the Single Cell 5' V(D)J assay:
- Peripheral Blood Mononuclear Cells (PBMCs)* - AllCells (Catalog # PB003F), cryopreserved, 15 million cells
- Normal Peripheral Blood Pan T Cells - AllCells (Catalog # PB009-1F) - Cryopreserved, 20 million cells, negatively isolated from mononuclear cells with magnetic beads
* Lower cell capture efficiency is expected since only 50-70% of PBMCs are T cells
Please see the 10x Genomics Demonstrated Protocol 'Fresh Frozen Human Peripheral Blood Mononuclear Cells for Single Cell RNA Sequencing' for general guidelines on cell thawing, washing and preparation: https://support.10xgenomics.com/single-cell-vdj/index/doc/demonstrated-protocol-fresh-frozen-human-peripheral-blood-mononuclear-cells-for-single-cell-rna-sequencing