For the Single Cell 3' and Single Cell 5' VDJ applications, the Eppendorf twin.tec 96-well PCR plates are recommended. We have also validated the following strip tubes to use in place of the twin.tec plate:
- TempAssure PCR 8-tube strip (USA Scientific) - Part Number (US) 1402-4700
- PCR Tubes 0.2 ml 8-tube strips (Eppendorf) - Part Number (US) 0030124286
- MicroAmp 8 Tube Strip (Thermo Fisher) - Part Number (US) N8010580 (tubes) and N8010535 (caps)
The PCR Tubes from Eppendorf are available for order in Europe - Part Number 0030124359
Note: The plastics other than those recommended here could negatively impacted the performance of the RT step and the integrity of emulsions resulting in an overall low cDNA yield after amplification. Also, plastics other than those recommended here may cause premature breaking of the GEMs before or during the RT reaction which will allow mRNAs from all cells and barcoded primers from all Gel Beads to pool. If this happens, single cell resolution of the expression analysis is lost.