Question: How are SPRIselect volumes calculated?
Answer: Solid Phase Reversible Immobilization (SPRI) beads selectively enrich DNA fragments according to the ratio of SPRIselect Reagent (beads) added to DNA solution.
For information on how SPRIselect Reagent volumes are calculated, please see the Troubleshooting section of the product User Guide.
For general information on the SPRIselect Reagent and DNA size selection, the following Technical Note may be helpful: SPRIselect:DNA Ratios Affect the Size Range of Library Fragments (https://support.10xgenomics.com/genome-exome/index/doc/technical-note-spriselectdna-ratios-affect-the-size-range-of-library-fragments)
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