Question: What is the fill volume on reagents?
Answer: The 10x Genomics' Library Kits and Gel Bead Kits include enough reagents for the specified number of reactions plus an additional 10% overage to ensure there is sufficient volume to set up master mixes. For example, when setting up a master mix for 2 reactions, the total volume of the master mix should equal 2.2x of the single reaction volume.
The reagents and Gel Beads have been tested up to 10 freeze-thaw cycles. We do not recommend pre-aliquoting the reagents, which would increase the chance of reagent loss through the multiple transfers.