Question: What sample types are compatible with the Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol?
Answer: The Isolation of Cells from FFPE Tissue Sections for Chromium Fixed RNA Profiling Demonstrated Protocol is compatible with FFPE blocks. A list of FFPE tissues tested using the Demonstrated Protocol is available here: Single Cell FFPE Tested Tissues.
If your tissue of interest is not present in this list, other tissues with similar characteristics may have been tested. This may give an indication of how a specific tissue type may perform with the Demonstrated Protocol.
It is important to note that FFPE preservation is not standardized and thus, some blocks may be more suited for molecular analysis as compared to others. From internal studies, blocks that performed well with the Visium CytAssist Spatial Gene Expression for FFPE assay are likely to perform well with the Fixed RNA Profiling assay. For untested FFPE blocks, users may wish to perform a pilot run before scaling up to a large number of samples.
Products: Fixed RNA Profiling