Question: Is it necessary to sequence the Gene Expression library in a Barcode Enabled Antigen Mapping (BEAM) experiment?
Answer: Yes, a Gene Expression library is required for analyzing BEAM data in Cell Ranger. If you are not interested in analyzing the gene expression profile in detail, you can perform shallow sequencing on the Gene Expression library. 5,000 reads per cell is a suggested starting point. The minimum Gene Expression sequencing depth needed for accurate cell calling may vary based on the sample type and data quality.
Products: Single Cell Immune Profiling, Barcode Enabled Antigen Mapping (BEAM)