Question: Why was the part number of the 5’ Feature Barcode Kit updated from 1000256 to 1000541?
Answer: The 5’ Feature Barcode Kit was updated to include a new primer required for our Barcode Enabled Antigen Mapping (BEAM) product. Specifically, “Feature cDNA Primers 5” was added to the 5’ Feature Barcode Kit. This primer is required during the cDNA amplification step in BEAM experiments.
If you are generating Cell Surface Protein libraries only (no BEAM libraries) you can use kit 1000256 or kit 1000541.
If you are generating BEAM libraries (with or without Cell Surface Protein libraries), kit 1000541 is required.
Products: Single Cell Immune Profiling, Barcode Enabled Antigen Mapping (BEAM)