Can I use alternative third-party reagents for Xenium?

Question: Can I use alternative third-party reagents for Xenium?

Answer: It is strongly recommended to use the third-party reagent part number listed in the Xenium In Situ Gene Expression and Xenium Analyzer Demonstrated Protocols and User Guides. Substituting untested third-party materials may adversely affect system performance. When the part numbers listed are not available, it may be possible to substitute additional reagents as low-risk alternatives as long as they are the meet the following criteria:

• Same molecular biology grade and chemical formulation
• Nuclease-free
• Same concentration and/or pH

Caution: Avoid alternative reagents that have ingredients/additives not found in the recommended products.

Here is a list reagents (from each protocol) that are considered to be low-risk to substitute a suitable alternative:
All DPs and UGs

Probe Hybridization, Ligation & Amplification (CG000582)

H&E Staining (CG000613)

Alternatives to the part numbers provided above have not been tested in-house and therefore we cannot guarantee assay and instrument performance and is not recommended. Please reach out to support@10xgenomics.com if a validated reagent cannot be obtained.

Product: Xenium In Situ Gene Expression